GEK1, a gene product of Arabidopsis thaliana involved in ethanol tolerance, is a d-aminoacyl-tRNA deacylase

GEK1, an Arabidopsis thaliana gene product, was recently identified through its involvement in ethanol tolerance. Later, this protein was shown to display 26% strict identity with archaeal d -Tyr-tRNA Tyr deacylases. To determine whether it actually possessed deacylase activity, the product of the GEK 1 open reading frame was expressed in Escherichia coli from a multi-copy plasmid. Purified GEK1 protein contains two zinc ions and proves to be a broad-specific, markedly active d -aminoacyl-tRNA deacylase in vitro . Moreover, GEK 1 expression is capable of functionally compensating in E. coli for the absence of endogeneous d -Tyr- tRNA Tyr deacylase. Possible connections between exposure of plants to ethanol/acetaldehyde and misaminoacylation of tRNA by d -amino acids are considered.